The effect of hypophysectomy on the glutathione-insulin transhydrogenase activity of rat liver.

نویسندگان

  • J H Thomas
  • A Jones
  • P Davey
  • J S Hutchinson
چکیده

Dodge, J., Mitchell, C. & Hanahan, D. (1963) Arch. Biochem. Biophys. 100, 119-130 Fahimi, H. D. & Karnovsky, M. J. (1964) J . Cell Biol. 23, 29A Goldman, R., Kedem, 0. & Katchalsky, E. (1968) Biochemistry 7 , 4518-4531 Goldman, R., Kedem, 0. & Katchalsky, E. (1971) Biochemistry 10, 165-172 Goldstein, L., Levin, Y. & Katchalsky, E. (1964) Biochemistry 3, 1913-1919 Hornby, W. E., Lilly, M. D. & Crook, E. M. (1968) Biochem. J. 107, 669-674 Kant, J. A. & Steck, T. L. (1973) J. Biol. Chem. 248, 8457-8464 McDaniel, C. F., Kirtley, M. E. & Tanner, M. J. A. (1974) J. Biol. Chem. 249,6478-6485 Schrier, S . L. (1967) Biochim. Biophys. Acta 135, 5991-598 Schrier, S. L., Junga, I. &Johnson, M. (1975) Life Sci. 17, 735-738 Wooster, M. S. & Wrigglesworth, J. M. (1976) Biochem. J. 153, 93-100

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Assay of Microsomal Membrane-bound Glutathione-insulin Transhydrogenase and Comparison with Protein

1. Inhibition of endogenous microsomal NADPH oxidase by CO enables membranebound glutathione-insulin transhydrogenase (EC 1.8.4.2) to be assayed conveniently by a linked assay involving NADPH and glutathione reductase (EC 1.6.4.2). 2. The specific activity of the enzyme in rat liver microsomal preparations is of the order of 1 nmol of oxidized glutathione formed/min per mg of membrane protein. ...

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عنوان ژورنال:
  • Biochemical Society transactions

دوره 4 4  شماره 

صفحات  -

تاریخ انتشار 1976